Electronic nose (E-nose) technology is composed from various types of sensor to analyses odorant molecules and produce some sort of signal then sent to the computer that use multivariate analysis to determine patterns of the data. This pattern recognition us used to determine the smaple is similar or different from another based on headspace volatiles. Electronic nose device used for detecting different bacteria cultured on media agar. In addition, multivariate methods: k-nearest neighbor (KNN), support vector machine (SVM) and neural network(NN) are used to determine the performance of bacterial compound. The study shows the performance of electronic nose (E-nose) for early screening for bacterial infection in human stomach.

Fish of Oreochromis niloticus (Linnaeus, 1758) were treated with different concentrations of Enterotoxigenic Escherichia coli (ETEC) as 103-105, 106 -107 and 109-1010 CFU/ml water. The investigation had conducted to detect experimentally the impact of E. coli toxins on Oreochromis niloticus fish throughout cells blood counts (WBCs, RBCs and Platelets), hemoglobin concentration and histopathological changes of muscles. The values of WBCs, RBCs, Platelets and hemoglobin ranged between (90.70 K/ml -7.10 K/ml), (2.00 m/ml - 0.53 m/ml), (21 K/ml - 3 K/ml) and (8.2 G/dl to 2.50 G/dl) for the hematological indices respectively. The concentration of WBCs, RBCs, Platelets and hemoglobin were lower than control in all the experiment phases. Histopathological changes of O. niloticus treated with E. coli included degeneration, necrosis, atrophy and inflammatory infiltration of WBCs. The results revealed mortality of fish in the highest concentration and high incidence of E. coli toxins in the muscles of O. niloticus induced damage in the muscle tissues and may transfer to the human

consumer and cause serious illness.