In this study, novel techniques have been proposed for the development of liposome formulations that will transfer the pDNA with athrombospondin gene, which has been shown to prevent tumor growth by inhibiting angiogenesis, to an active‚ safe and resistant cell. Ouraim was to develop liposome formulations that can directly affect angiogenic tumor cells without harming vasculogenesis exhibiting cells.F5 and F9 coded PEGylated liposomes were selected for investigation of their physicochemical and physicopharmaceutical properties. Positivelycharged lipoplexes size were around 150 nm and they encapsulated the plasmid at a rate of 35 %. Furthermore, ‚ F5 coded PEGylatedliposomes investigated the applicability for gene transfection efficiency at MCF-7 (breast cancer epithelial) cells. Accordingly, the ‚ F5 codedformulation can be further used as a gene delivery system in the in vivo studies to obtain preliminary findings on anti-cancer treatment bythe anti-angiogenesis approach.
Biocore Publishing Group
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