Kemal Gokce, Seker Dag*
In this study, glutathione peroxidase activity (GPx) (EC:22.214.171.124) and amount of reduced glutathione (GSH) and oxidized glutathione disulfite was investigated in blood samples of who diagnosed with colon cancer individuals whose ages between 18-75. Erythrocyte were isolated immediately from patient and control groups after 3 ml of blood samples were taken. Amount of hemoglobin was determined in presence of cynanide with conversion of methemoglobin to cynomethemglobin at 540 nm by standart curve. GPx activity was determined by oxidation of NADPH at 340 nm spectrophotometrically. Amounts of GSH and GSSG were determined at 412 nm by using standard curve.
While GPx activity of stomach cancer patients (5,80 ± 1,40 U/gHb), contcentration of GSH (7,66 ± 1,19 nmol/gHb) and GSH/GSSG ratio (1,29 ± 0,46) significantly decreased comparing to control group (GPx: 10,52 ± 2,22 U/gHb, GSH: 11,43 ± 1,90 nmol/gHb, GSH/ GSSG: 3,86 ± 1,30), amount of GSSG of patient group (6,33 ± 1,18 nmol/gHb) significantly increased comparing to control group (3,09 ± 0,48 nmol/gHb). These results suggested that antioxidant/oxidant system balance abolished in patients who has stomach cancer.
Shajrat Mir, Parveen Jamal*, Md Zahangir Alama, Aarif Bashir Mir, Abdul Haseeb Ansari
Biosurfactants are surface active agents produced naturally by a wide variety of microorganisms, which include different strains of bacteria, fungi and yeast. Biosurfactants, also known as microbial surfactants, are amphiphilic compounds. It is the amphiphilic nature of biosurfactants that makes them excellent foaming, emulsifying and dispersing agents. The .0surfactants or biosurfactants increase the surface area of the water-insoluble hydrophobic entities. They are surpassing their chemical counterparts. This can be attributed to diversity, high biodegradability, less toxicity, greater stability and ecological acceptance of biosurfactants in comparison with the chemically prepared surfactants. However, currently, production of biosurfactants is a very expensive process mainly because of the costly synthetic media required by the microorganisms to survive and grow. Therefore, much stress is being put on augmenting researching on cheap or cost free and nutrient rich renewable wastes to be used as substrates for various microbes to grow and produce biosurfactants. Research for new strains with high productivity is a challenge for the widespread application of microbial surfactants. This review focuses on the extensive evaluation of biosurfactants and their application on commercial scale.
Biocore Publishing Group
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