We aimed to determine the serological profiles of HBV among HIV-infected patients in Istanbul A multicentre retrospective study has been conducted by ACTHIV-IST study group. Demographic and laboratory data were collected from the patients’ files. A total of 567 HIV/AIDS patients were included in this study. Mean age was 38.5 years ± 11.2 and 81.5% were male. Serological profiles: 8.4% had current HBV infection, 9.3% had been vaccinated and 16.8 % had past infection. Parameters associated with current infection were lower CD4 counts (p <0.05). Of 58 patients with isolated anti-HBc , 29 were tested for serum HBV DNA and 3 of them were positive. Serological profiles of HBV must be assesed among HIV-infected patients and vaccination must be offered.