In this research, an unrivaled water-based eco-friendly nanopackage was designed including ZnO@modified-CNTs, alumina@silica, ZnO quantum dot NPs, pharmaceutical binder, viscosity control, emulsifier and dispersing agents. Characterizing the final nanofluid, the SEM, FTIR, XRD and UV-Vis were applied. The inhibition of cell growth for this nanopackage was evaluated by MTT assay against A549, Huh-7, A-375, KB44, MCF-7, and HT29 cell lines after incubation period of 24 and 48 h. Interestingly, the nanopackage treatments during 48 h showed no toxicity against normal human foreskin fibroblast cells (HFF-2) as a control. The obtained values of IC50 (µg/ml) were determined as follow: A549 cells (60), Huh-7 cells (75), A-375 cells (90), KB44 cells (65), MCF-7 cells (50), and HT29 cells (75) after 48 h of exposure with comparison of 195 (µg/ml) for control, respectively (P<0.05). The nanoproduct indicated very potential as selective and smart cytotoxic agent for inhibiting the growth, proliferation and eradication of these current cancer cells. Strong diffusion, smart surface modification, structural defects and ROS process can possess extravagant roles in oxidation-reduction chemical reactions related to cancer therapy and kinds of virus infections.
Vaccination of susceptible animals against foot-and-mouth disease (FMD) is a well-established strategy to combat the disease. The protective immune response induced by vaccines can vary according to the kinds of adjuvants. The advance in nanotechnology has enabled us to utilize particles in the size of the Nano scale. There for using novel immune adjuvants has an auxiliary role on the amplification of immune responses. Many investigators agree the size of the adjuvant particles is crucial to their adjuvant activities. The main aim of this study is to evaluate the effect of Silver nitrate nanoparticles (AgNPs) 5-10 nm particle size as an adjuvant in the polyvalent foot and mouth disease vaccine (containing FMD viruses O / PanAsia2, A/Iran 05, SAT2/VII/Lib-12 (SAT2/ Lib) and SAT2/VII/Ghb-12(SAT2/Ghb). A imprehensive immunological study was conducted in three calve groups vaccinated ubcutaneously with three formulae of polyvalent FMD where group (A) was vaccinated vaccine formula with AgNPs adjuvant, group (B) the vaccine formula adjuvanted with both Montanid ISA 206 oil and AgNPs, while group (C) the vaccine formula with Montanid ISA 206 oil adjuvant. A forth calve group kept without vaccination as control. The humeral and cellular immune responses were monitored in all calve groups. The obtained results indicated that the incorporation of AgNPs inactivated FMD vaccine induces an increase of the specific protective immune response. The higher and longer period of immune responses found in calves vaccinated with both oil and AgNPs adjuvanted vaccine up to 40 weeks. In contrast, those vaccinated with AgNPs and with oil vaccine showed protected immunity up to 32 and 36 weeks respectively. So it could be recommended to use both oil and AgNPs as an adjuvant to polyvalent FMD vaccine to provide adequate long-lasting immunity in vaccinated calves.
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